Dengue Virus (DENV) Antiviral Services
Antiviral assays against Dengue virus are performed to determine the inhibitory activity of small-molecule compounds and neutralizing antibodies for vaccine design. In vitro replication and infectivity assays against DENV-2 (16681 and TSV01) are routinely performed in monolayers of Vero and C6/36 cells. Several readouts are used to monitor virus replication, including specific ELISA assays and flow cytometry determination of intracellular viral antigens (e.g. capsid protein). These assays allow rapid and sensitive detection of Dengue virus replication in human and mosquito cell lines. Antiviral assays against other DENV strains are under development.
Viral pseudotypes carrying DENV envelope can be used as a surrogate assay to evaluate Dengue virus entry inhibitors. Studies with HIV pseudotypes are commonly used in our laboratories for this purpose.
For additional information about Dengue antiviral services call us at (858) 677-9315 or contact us at antivirals@retrovirox.com
To see more information about antiviral assays offered against other viruses click on the following links:
